CCAPD: Alignment of Cellulase F (glycosyl hydrolase 10; endo-1,4-beta-xylanase)

10 20 30 40 50 60 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS ----------------------------------------------vhlkt--lag-sav * PENICILLIUM_SIMPLICISSIMUM ------------------------------------------------------------ * THERMOASCUS_AURANTIACUS ----------------------------------------------vrpti--llt-sll * 1TUX0_Thermoascus_aurantiacus ------------------------------------------------------------ * Agaricus_bisporus -----------------------------------------------ryas--vea-sfd * Aspergillus_aculeatus ----------------------------------------------vqika--aal-avl * Aspergillus_flavus ----------------------------------------------vhlks--lag-ill * Aspergillus_niger ----------------------------------------------vqika--aal-aml * Fusarium_oxysporum htfsvllalapvsalaqapiwgqcggngwtgattcasglkcekin-dwyyqcvpgsggse * Gibberella_zeae hflglvvafapaalaqsaiwgqcggtgwsgsttcqsglkcekind-fyyqc--ipg-sdn * Neurospora_crassa -kaslmml--------------------------------------laplaamaaptsdn * Penicillium_chrysogenum -----------------------------------------------ipnitqlktaalv * Trichoderma_reesei -kanvilcl-------------------------------------laplvaalptetih ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS ---------------------------------------------r---------nvvrk B 1R86A_Bacillus_stearothermophilus ------------------------------------------------------------ ================================================================================================ B STREPTOMYCES_COELICOLOR ----------------------------------------------g------syalprs B STREPTOMYCES_OLIVACEOVIRIDIS ------------------------------------------------------------ B Cellulomonas_fimi ------------------------------------------------------htklha B Nonomuraea_flexuosa ----------------------------------------------g------vnafprp B Streptomyces_avermitilis ----------------------------------------------g------fhalprs B Streptomyces_thermoviolaceus ----------------------------------------------g------shalprp ================================================================================================ B PSEUDOMONAS_AERUGINOSA ---------------------------------------------g-------------- B 1W3HB_Pseudomonas_aeruginosa ---------------------------------------------g-------------- ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM ---------------------------------------------r-------------- ================================================================================================ Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 70 80 90 100 110 120 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSVnxxxxxxxxgkkyfgtxxa ================================================================================================ * EMERICELLA_NIDULANS f---as-la--taavl--p-------r------qs-a--SLNdlfv-a-aGKsYFGT--c * PENICILLIUM_SIMPLICISSIMUM ---------------------------------qasv--SIDakfk-a-hGKKYlGT--i * THERMOASCUS_AURANTIACUS l---ap-fa--aaspilee-------r------qaaq--SVDqlik-a-rGKvYFGv--A * 1TUX0_Thermoascus_aurantiacus ---------------------------------aaaq--SVDqlid-a-rGKvYFGv--A * Agaricus_bisporus m---ap-mi--hlpgrssg-------r------pgqv--lVltvnslp-rGKKFFGa--A * Aspergillus_aculeatus f---as-nv--lanpi-ep-------r------qasv--SIDakfk-a-hGKKYlGT--i * Aspergillus_flavus y---ts-lc--iass-----------q------qapa--SINnafv-a-kGKKYFGT--c * Aspergillus_niger f---as-hv--lsepi-ep-------r------qasv--SIDskfk-a-hGKKYlGn--i * Fusarium_oxysporum pqpsstqgggtpqptggn---------------sggt--gLDakfk-a-kGKQYFGT--e * Gibberella_zeae g---gg-tt--pdpgt--p-------spgngnada-t--gLDakir-a-kGKiYFGT--e * Neurospora_crassa lepr-----------------------------qasq--SIDalmk-a-kGKlYFGa--c * Penicillium_chrysogenum m--lfagqalsgpvesr----------------qase--SIDakfk-a-hGKKYlGn--i * Trichoderma_reesei l---dpela--alranltertadlwdr------qasq--SIDqlik-r-kGKlYFGT--A ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS pltiglaltlllpmgmtatsaknadsyakkphisalnapqLDqryk-n-e--ftiGa--A B 1R86A_Bacillus_stearothermophilus -----------------------------kphisalnapqLDqryk-n-e--ftiGa--A ================================================================================================ B STREPTOMYCES_COELICOLOR gvrr---sirvlllalvvgvlgtatalia-ppgahaaesTLgaaaa-qsg--RYFGT--A B STREPTOMYCES_OLIVACEOVIRIDIS ------------------------------------aesTLgaaaa-qsg--RYFGT--A B Cellulomonas_fimi tprh---gwrpraaalaataglvlttfaa-istpaqaasTLgasaa-ekg--RYYGT--A B Nonomuraea_flexuosa garrftgglyralaaatvsvvgvvtaltv-tqpasaaasTLaegaa-qhn--RYFGv--A B Streptomyces_avermitilis agrp---klrallpallvgalgfagavaapppqaqaaesTLgaaaa-qsg--RYFGv--A B Streptomyces_thermoviolaceus alrq---rirg-gcgrgrgvlglgatlst-pptahaaesTLgaaaa-qsg--RYFGT--A ================================================================================================ B PSEUDOMONAS_AERUGINOSA ------------------------------------n--gLasla--d----fpiGvavA B 1W3HB_Pseudomonas_aeruginosa ------------------------------------n--gLasla--d----fpiGvavA ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM -------iitefpgqgdvqtpnpsvtptqtpiptisg-naLrdyae-arg--ikiGT--c ================================================================================================ Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSVnxxxxxxxxgkkyfgtxxa 130 140 150 160 170 180 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxdxxxlxxNxNxxxxxxIIQxEFgxVTxENsMKFDATEpnqgKFSFSxaDrLVNWA ================================================================================================ * EMERICELLA_NIDULANS s----DqalL--q-Ns-qneaIVasQFGvITpENSMKWDAlEPSQGnFgWSgADyLVDYA * PENICILLIUM_SIMPLICISSIMUM g----DqytL--t-kntknpaIIKaDFGqLTpENSMKWDATEPNRGQFTFSgsDyLVNFA * THERMOASCUS_AURANTIACUS t----DqnrL--t-tg-knaaIIQanFGqVTpENSMKWDATEPSQGnFNFagADyLVNWA * 1TUX0_Thermoascus_aurantiacus t----DqnrL--t-tg-knaaIIQaDFGqVTpENSMKWDATEPSQGnFNFagADyLVNWA * Agaricus_bisporus a----DqntI--N-ia-anqaLLisDFGaVTpENSMKWDATEPNRGQFNFggADfLVNWA * Aspergillus_aculeatus g----DqytL--NkNa-ktpaIIKaDFGqLTpENSMKWDATEPNRGQFSFSgsDyLVNFA * Aspergillus_flavus a----DqgtL--S-Dg-tnsgIIKaDFGqLTpENSMKWDATEPSQGKFSFSgADyLVNYA * Aspergillus_niger g----DqytL--tkNs-ktpaVIKaDFGaLTpENSMKWDATEPSRGQFSFSgsDyLVNFA * Fusarium_oxysporum i----DhyhL--N-Nnpli-nIVKaQFGqVTcENSMKWDAiEPSRnsFTFSnADKVVDFA * Gibberella_zeae i----DhyhL--S-Nn-plinIVKkDFGqVTnENSMKWDAiEPSRGQFTFSnADKVVDFA * Neurospora_crassa t----DqgrL--t-Sg-knavILQnnFGqVTpENSMKWDsiQPqRGQYNFgqADyLVNWA * Penicillium_chrysogenum a----DqgtL--N-gnpktpaIIKanFGqLSpENSMKWDATEPSQGQFSFagsDyfVeFA * Trichoderma_reesei t----DrglL--q-re-knaaIIQaDlGqVTpENSMKWqslEnNQGQlNWgdADyLVNFA ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS v---epyqlq--N-e--kdvqMLKrhFnsIvaENvMKpisiQPeEGKFNFeqADRIVkFA B 1R86A_Bacillus_stearothermophilus v---epyqlq--N-e--kdvqMLKrhFnsIvaENvMKpisiQPeEGKFNFeqADRIVkFA ================================================================================================ B STREPTOMYCES_COELICOLOR i---asgrls--D-S--tytsIagrEFnmVTaENeMKiDATEPqRGQFNFSsADRVyNWA B STREPTOMYCES_OLIVACEOVIRIDIS i---asgklg--D-S--ayttIasrEFnmVTaENeMKiDATEPqRGQFNFSagDRVyNWA B Cellulomonas_fimi i---aagrmg--D-S--tymtIanrEFnmITaENeMKmDATEPSQGRFTFTngDRIVNWA B Nonomuraea_flexuosa i---aanrlt--D-S--vytnIanrEFnsVTaENeMKiDATEPqQGRFdFTqADRIyNWA B Streptomyces_avermitilis i---aanrls--D-S--tyasIanrEFnsVTaENeMKiDATEPqRGQFNFqnADRIyNWA B Streptomyces_thermoviolaceus i---aagrls--D-S--tytsIasrEFnmVTaENeMKiDATEPqRGQFdFSagDRVyNWA ================================================================================================ B PSEUDOMONAS_AERUGINOSA asggnadift--S-S--arqnIVRaEFnqITaENiMKmsymy-SgsnFSFTnsDRLVSWA B 1W3HB_Pseudomonas_aeruginosa asggnadift--S-S--arqnIVRaEFnqITaENiMKmsymy-SgsnFSFTnsDRLVSWA ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM v---nypfynnsD-p--tynsILQrEFsmVvcENeMKFDAlQPrQnvFdFSkgDQLLaFA ================================================================================================ Consensus xxxxxdxxxlxxNxNxxxxxxIIQxEFgxVTxENsMKFDATEpnqgKFSFSxaDrLVNWA 190 200 210 220 230 240 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxNGKxIRGHTLVWHSxxQLPxWMxxxixxnxxxxxxxxxxxxxxxxsxLxxVMKNHIST ================================================================================================ * EMERICELLA_NIDULANS tqHnKkVRGHTLVWHS--QLPsWVs-sI-gD--------------anT-LrsVMtNHINe * PENICILLIUM_SIMPLICISSIMUM qsNGKlIRGHTLVWHS--QLPgWVs-sI-tD--------------knT-LisVLKNHITT * THERMOASCUS_AURANTIACUS qqNGKlIRGHTLVWHS--QLPsWVs-sI-tD--------------knT-LtnVMKNHITT * 1TUX0_Thermoascus_aurantiacus qqNGKlIRGHTLVWHS--QLPsWVv-sI-tD--------------knT-LtnVMKNHITT * Agaricus_bisporus tsHGKmIRGHTfVWHS--QLPgWVs-sI-nD--------------rtT-LtsVIQNHIST * Aspergillus_aculeatus qsNGKlIRGHTLVWHS--QLPsWVq-sI-yD--------------kgT-LiqVMQNHIaT * Aspergillus_flavus atNnKlIRGHTLVWHS--QLPsWVq-gI-tD--------------knT-LtsVLKNHITT * Aspergillus_niger qsNnKlIRGHTLVWHS--QLPsWVq-aI-tD--------------knT-LieVMKNHITT * Fusarium_oxysporum tqNGKlIRGHTLLWHS--QLPqWVq-nI-nD--------------rsT-LtaVIENHVkT * Gibberella_zeae qaNGKkIRGHTLLWyS--QLPqWVk-nI-rD--------------raT-MtsVIENHVkT * Neurospora_crassa taNnKtIRGHTfLWHS--QLagWVn-nI-rD--------------knq-LttVLQeHITT * Penicillium_chrysogenum etNGKlIRGHTLVWHS--QLPsWVs-sI-tD--------------ktT-LtdVMKNHITT * Trichoderma_reesei qqNGKsIRGHTLIWHS--QLPaWVn-nI-nN--------------adT-LrqVIRtHVST ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS kaNGmdIRfHTLVWHS--QVPqWff-ldkegkpmvnetdpvkreqnkqlLlkrLEtHIkT B 1R86A_Bacillus_stearothermophilus kaNGmdIRfHTLVWHS--QVPqWff-ldkegkpmvnetdpvkreqnkqlLlkrLEtHIkT ================================================================================================ B STREPTOMYCES_COELICOLOR vqNGKqVRGHTLaWHS--QqPgWMqslsg------------------SaLrqaMiDHINg B STREPTOMYCES_OLIVACEOVIRIDIS vqNGKqVRGHTLaWHS--QqPgWMqslsg------------------StLrqaMiDHINg B Cellulomonas_fimi lsNGKrVRGHTLaWHa--QqPgWMqsmsg------------------SaLrnaLiNHVTq B Nonomuraea_flexuosa rqNGKqVRGHTLaWHS--QqPqWMqnlsg------------------qaLrqaMiNHIqg B Streptomyces_avermitilis vqNGKqVRGHTLaWHS--QqPgWMqslsg------------------SaLrqaMiDHINg B Streptomyces_thermoviolaceus vqNGKeVRGHTLaWHS--QqPyWMqslsg------------------SdLrqaMiDHINg ================================================================================================ B PSEUDOMONAS_AERUGINOSA aqNGQtVhGHaLVWHpsyQLPnWas-d-----------------snaN-frqdfarHIdT B 1W3HB_Pseudomonas_aeruginosa aqNGQtVhGHaLVWHpsyQLPnWas-d-----------------snaN-frqdfarHIdT ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM erNGmqMRGHTLIWHN--QnPsWLtng--nw--------------nrdsLlaVMKNHITT ================================================================================================ Consensus xxNGKxIRGHTLVWHSxxQLPxWMxxxixxnxxxxxxxxxxxxxxxxsxLxxVMKNHIST 250 260 270 280 290 300 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus LMxrYKxxgkIxxWDVVNEvFxedgsxxxxxxxxxxxxxrxxxNTvFxQVIxGDDFIKIA ================================================================================================ * EMERICELLA_NIDULANS VVgRYK--GKImhWDVVNEIFnEDGT-------f-----R---NSVFynLL-GEDFVRIA * PENICILLIUM_SIMPLICISSIMUM VMtRYK--GKIyaWDVLNEIFnEDGS-------l-----R---NSVFynVI-GEDYVRIA * THERMOASCUS_AURANTIACUS LMtRYK--GKIraWDVVNEaFnEDGS-------l-----R---qTVFlnVI-GEDYIpIA * 1TUX0_Thermoascus_aurantiacus IMtRYi--GKIraWDVVNEaFnEDGS-------l-----R---qTVFnnVI-GEDYIpIA * Agaricus_bisporus LggRYR--GKIyaWDVcNEIFnEDGS-------i-----R---qSVFsnVL-GEsFVtIA * Aspergillus_aculeatus VMqRYK--GKVyaWDVVNEIFnEDGS-------l-----R---qShFynVI-GEDYVRIA * Aspergillus_flavus VMnRYK--GKVyaWDVVNEIFnEDGT-------l-----R---SSVFynVL-GEDFVRIA * Aspergillus_niger VMqhYK--GKIyaWDVVNEIFnEDGS-------l-----R---DSVFyKVI-GDDYVRIA * Fusarium_oxysporum MVtRYK--GKIlqWDVVNEIFaEDGN-------l-----R---DSVFsRVL-GEDFVgIA * Gibberella_zeae VVtRYK--GKIlhWDVVNEIFaEDGN-------m-----R---NSeFyQVL-GEDFVgIA * Neurospora_crassa VMtRYK--GKIygYDVaNEIFnEDGS-------l-----R---SSIFyQVL-GEDmVsIA * Penicillium_chrysogenum VMkQYK--GKLyaWDVVNEIFeEDGT-------l-----R---DSVFsRVL-GEDFVRIA * Trichoderma_reesei VVgRYK--GKIraWDVVNEIFnEDGT-------l-----R---SSVFsRLL-GEEFVsIA ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS IVeRYKdd--IkyWDVVNEVvg----d-----dg-----K-lrNSpWyQIa-GiDYIKVA B 1R86A_Bacillus_stearothermophilus IVeRYKdd--IkyWDVVNaVvg----d-----dg-----K-lrNSpWyQIa-GiDYIKVA ================================================================================================ B STREPTOMYCES_COELICOLOR VMahYKgk--IvqWDVVNEaFa----dgs---------sgarrDSnlqRs--GNDWIEVA B STREPTOMYCES_OLIVACEOVIRIDIS VMghYKgk--IaqWDVVNEaFs----ddg---------sggrrDSnlqRt--GNDWIEVA B Cellulomonas_fimi VasyYRgk--VyaWDVVNEaFa----ddg---------rgsrrDSnlqRt--GNDWIEaA B Nonomuraea_flexuosa VMsyYRgk--IpiWDVVNEaFe----dgn---------sgrrcDSnlqRt--GNDWIEVA B Streptomyces_avermitilis VMahYKgk--IaqWDVVNEaFa----dgs---------sgarrDSnleRt--GsDWIEVA B Streptomyces_thermoviolaceus VMnhYKgk--IaqWDVVNEaFe----dgn---------sgarrDSnlqRt--GNDWIEVA ================================================================================================ B PSEUDOMONAS_AERUGINOSA VaahFagq--VksWDVVNEalf----dsaddpdgrgsang-yrqSVFyRqfgGpEYIdeA B 1W3HB_Pseudomonas_aeruginosa VaahFagq--VksWDVVNEalf----dsaddpdg-gsang-yrqSVFyRqfgGpEYIdeA ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM VMthYKgk--IveWDVaNEcmd----d-----sg-----nglrSSIWrnVI-GqDYLdyA ================================================================================================ Consensus LMxrYKxxgkIxxWDVVNEvFxedgsxxxxxxxxxxxxxrxxxNTvFxQVIxGDDFIKIA 310 320 330 340 350 360 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus FQtARAADpxSAKLYINDYNLDNxxxxxAKxtxxxgIxSxVKQFxxqGVPIDGIGsQSHL ================================================================================================ * EMERICELLA_NIDULANS FETARAADP-dAKLYINDYNLDS--asyAK-T-q-aMaSyVKKWlaEGVPIDGIGSQaHy * PENICILLIUM_SIMPLICISSIMUM FETARsvDP-NAKLYINDYNLDS--agysK-v-n-GMvShVKKWlaaGIPIDGIGSQTHL * THERMOASCUS_AURANTIACUS FQTARAADP-NAKLYINDYNLDS--asypK-T-q-aIvNrVKKWraaGVPIDGIGSQTHL * 1TUX0_Thermoascus_aurantiacus FRTARAADP-NAKLYINDYNLDS--askpK-T-s-aIvkrVKKWraaGVPIDGIGSQTHL * Agaricus_bisporus FQaARsADP-NAKLYINDYNLDS--nn-AK-v-q-GMvalVKRqnanGriIDGIGTQmHL * Aspergillus_aculeatus FETARAvDP-NAKLYINDYNLDS--asypK-l-t-GLvNhVKKWvaaGVPIDGIGSQTHL * Aspergillus_flavus FETARAADP-qAKLYINDYNLDS--anygK-T-t-GLaNhVKKWiaQGIPIDGIGSQTHL * Aspergillus_niger FETARAADP-NAKLYINDYNLDS--asypK-l-a-GMvShVKKWieaGIPIDGIGSQTHL * Fusarium_oxysporum FRaARAADP-aAKLYINDYNLDk--sdyAK-l-trGMvahVnKWiaaGIPIDGIGSQgHL * Gibberella_zeae FRaARAADP-aAKLYINDYNLDi--anyAKvT-r-GMvdhVnKWvsQGIPIDGIGSQaHL * Neurospora_crassa FKaARAADP-NAKLYINDYNLDS--pnyAK-vtt-GMiqhVnKWvaaGVPIDGIGTQgHI * Penicillium_chrysogenum FETAReADP-eAKLYINDYNLDS--atsAK-l-q-GMvShVKKWiaaGVPIDGIGSQTHL * Trichoderma_reesei FRaARdADP-SARLYINDYNLDr--anygK-v-n-GLkTyVsKWisQGVPIDGIGSQSHL ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS FQaARkyggdNiKLYMNDYNtEv--e--pK-r-t-aLyNlVKQlkeEGVPIDGIGhQSHI B 1R86A_Bacillus_stearothermophilus FQaARkyggdNiKLYMNDYNtEv--e--pK-r-t-aLyNlVKQlkeEGVPIDGIGhQSHI ================================================================================================ B STREPTOMYCES_COELICOLOR FRTARAAD-pSAKLcyNDYNVENwtw--AK-T-q-aMyNmVRdFkqRGVPIDcVGfQSHf B STREPTOMYCES_OLIVACEOVIRIDIS FRTARAAD-paAKLcyNDYNIENwtw--AK-T-q-GVyNmVRdFkqRGVPIDcVGfQSHf B Cellulomonas_fimi FRaARAAD-pNAKLcyNDYNtDNwsh--AK-T-q-GVyNmVKdFkaRGVPIDcVGfQaHf B Nonomuraea_flexuosa FRTARqgD-pSAKLcyNDYNIENwna--AK-T-q-aVyNmVRdFksRGVPIDcVGfQSHf B Streptomyces_avermitilis FRTARAAD-pSAKLcyNDYNVENwtw--AK-T-q-aMyrmVKdFkqRGVPIDcVGfQSHf B Streptomyces_thermoviolaceus FRTARAAD-pSAKLcyNDYNIENwtw--AK-T-q-aVyNmVRdFkqRGVPIDcVGfQSHf ================================================================================================ B PSEUDOMONAS_AERUGINOSA FRrARAAD-pTAELYyNDFNtEe--ng-AK-T-t-aLvNlVQRllnnGVPIDGVGfQmHV B 1W3HB_Pseudomonas_aeruginosa FRrARAAD-pTAELYyNDFNtEe--ng-AK-T-t-aLvNlVQRllnnGVPIDGVGfQmHV ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM FRyAReAD-pdAlLFyNDYNIEDl-g--pK-S-n-aVfNmIKsmkeRGVPIDGVGfQcHf ================================================================================================ Consensus FQtARAADpxSAKLYINDYNLDNxxxxxAKxtxxxgIxSxVKQFxxqGVPIDGIGsQSHL 370 380 390 400 410 420 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus SxxxxxxxxxxxxxxssxxxxALqxLAxxxxxxEVAITELDVxxxxxxxxxxxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS S-sshwss-------Tea-agALssLAntgv-sEVAITELDIag---------------- * PENICILLIUM_SIMPLICISSIMUM g-a--gag-------Sav-agALnaLAsagt-kEIAITELDIag---------------- * THERMOASCUS_AURANTIACUS S-a--gqg-------aSv-lqALplLAsagt-pEVAITELDVag---------------- * 1TUX0_Thermoascus_aurantiacus S-a--gqg-------aSi-daALpnLAsagt-pEVAITELDIag---------------- * Agaricus_bisporus g-p--ggg-------Sga-qaAItaLAgagt--ELAITELDIqn---------------- * Aspergillus_aculeatus S-a--gag-------aav-sgALnaLAgagt-kEVAITELDIag---------------- * Aspergillus_flavus S-a--ggs-------Sgv-kgALntLAasgv-sEVAITELDIag---------------- * Aspergillus_niger S-a--ggg-------agi-sgALnaLAgagt-kEIAVTELDIag---------------- * Fusarium_oxysporum a-a--psgw-n-pa-Sgv-paALRaLAasda-kEIAITELDIsg---------------- * Gibberella_zeae akpggwnp-------aSgfpaALKvLAganv-kEVAITELDIdg---------------- * Neurospora_crassa N-a--ggg-------SNl-aaAIKaLAsanv-kEVAVTELDIqg---------------- * Penicillium_chrysogenum g-a--gag-------aaa-sgALnaLAsagt-eEVAVTELDIag---------------- * Trichoderma_reesei S-g--ggg-------Sgt-lgALQqLAtvpv-tELAITELDIqg---------------- ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS q-igw--p-s-e---aei-ektInmfAal--glDnqITELDVsmygwppr----ayptyd B 1R86A_Bacillus_stearothermophilus q-igw--p-s-e---aei-ektInmfAal--glDnqITaLDVsmygwppr----ayptyd ================================================================================================ B STREPTOMYCES_COELICOLOR N-sgsp-y-n-----SNf-rttLQnfAal--gvDVAITELDIq----------------- B STREPTOMYCES_OLIVACEOVIRIDIS N-sgsp-y-n-----SNf-rttLQnfAal--gvDVAITELDIq----------------- B Cellulomonas_fimi N-sgnp-v-p-----SNy-httLQnfAdl--gvDVqITELDIe----------------- B Nonomuraea_flexuosa N-sgnp-y-n-----pNf-rttLQqfAal--gvDVeVTELDIe----------------- B Streptomyces_avermitilis N-sgsp-y-n-----SNf-rttLQtfAal--gvDVAITELDIq----------------- B Streptomyces_thermoviolaceus N-sgsp-y-d-----SNf-rttLQsfAal--gvDVAITELDIq----------------- ================================================================================================ B PSEUDOMONAS_AERUGINOSA m-ndy--p-s-i---aNi-rqAMQkIvalsptlKIkITELDVrlnnpydgnssndytnrn B 1W3HB_Pseudomonas_aeruginosa m-ndy--p-s-i---aNi-rqAMQkIvalsptlKIkITELDVrlnnpydgnssndytnrn ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM i-ng---m-s-peylaSi-dqnIKryAei--gviVsfTEIDIripqsenp----ataf-- ================================================================================================ Consensus SxxxxxxxxxxxxxxssxxxxALqxLAxxxxxxEVAITELDVxxxxxxxxxxxxxxxxxx 430 440 450 460 470 480 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxAsxSxxxNYxxVLxaCLxxxxxxxxKCVGITMWGIxDxNSWRxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS ----------AasS---DYlnLLnACLneq-----KCVGITVWGVsDkDSWRas------ * PENICILLIUM_SIMPLICISSIMUM ----------ASsT---DYvnVVnACLnqa-----KCVGITVWGVaDpDSWRss------ * THERMOASCUS_AURANTIACUS ----------ASsT---DYvnVVnACLnvs-----sCVGITVWGVaDpDSWRas------ * 1TUX0_Thermoascus_aurantiacus ----------ATsT---DYvdVVnACLdvd-----sCIGITVWGVaDpDSWRas------ * Agaricus_bisporus ----------ASsS---DYvaVVnACLnqp-----aCVsITtWGVaDiNSWRsg------ * Aspergillus_aculeatus ----------ASsT---DYvnVVkACLnqp-----KCVGITVWGVaDpDSWRss------ * Aspergillus_flavus ----------ASsN---DYvnVVeACLevs-----KCVGITVWGVsDkNSWRsa------ * Aspergillus_niger ----------ASsT---DYveVVeACLdqp-----KCIGITVWGVaDpDSWRss------ * Fusarium_oxysporum ----------ASaN---DYltVMnACLavp-----KCVGITVWGVsDkDSWRpg------ * Gibberella_zeae ----------AaaN---DYvtVVnsCLttp-----KCVGITVWGVsDkDSWRse------ * Neurospora_crassa ----------nNag---DYatItkgCLsep-----KCVGITVWGVrDsDSWRpn------ * Penicillium_chrysogenum ----------ATsT---DYvdVVnACLdqp-----KCVGITVWGVaDpDSWRad------ * Trichoderma_reesei ----------AptT---DYtqVVqACLsvs-----KCVGITVWGIsDkDSWRas------ ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS --aipkqkfldqaa---rYdrLfklyek--lsd--KisnVTfWGIaDnHTWldsradvyy B 1R86A_Bacillus_stearothermophilus --aipkqkfldqaa---rYdrLfklyek--lsd--KisnVTfWGIaDnHTWldsradvyy ================================================================================================ B STREPTOMYCES_COELICOLOR ---------gApaS---tYanVtndCLa--vs---RCLGITVWGVrDsDSWRseq----- B STREPTOMYCES_OLIVACEOVIRIDIS ---------gASsS---tYaaVtndCLa--vs---RCLGITVWGVrDtDSWRsgd----- B Cellulomonas_fimi ---------gsgsSqaqqYqgVVqACLa--vs---RCtGITVWGVrDtDSWRasg----- B Nonomuraea_flexuosa ---------nApaq---tYasVIrdCLa--vd---RCtGITVWGVrDsDSWRsyq----- B Streptomyces_avermitilis ---------gASpN---tYaaVVndCLa--vs---RCLGVTVWGVrDmDSWRssd----- B Streptomyces_thermoviolaceus ---------gASpT---tYanVVndCLa--vs---RCLGITVWGVrDtDSWRsgd----- ================================================================================================ B PSEUDOMONAS_AERUGINOSA dcavscagldrqka---rYkeIVqAyLe--vvppgRrgGITVWGIaDpDSWlythq---- B 1W3HB_Pseudomonas_aeruginosa dcavscagldrqka---rYkeIVqAyLe--vvppgRrgGITVWGIaDpDSWlythq---- ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM ---------qvqaN---NYkeLMkiCLa--np---nCntfvMWGftDkyTWipgtfpgyg ================================================================================================ Consensus xxxxxxxxxxAsxSxxxNYxxVLxaCLxxxxxxxxKCVGITMWGIxDxNSWRxxxxxxxx 490 500 510 520 530 540 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxSPLLFNxNFNPKxAYSAIVDxxxlxxxxxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS -----------------------dSPLLFDgNYqPKdAYNAIVN--aLs----------- * PENICILLIUM_SIMPLICISSIMUM -----------------------sSPLLFDgNYNPKaAYNAIaN--aL------------ * THERMOASCUS_AURANTIACUS -----------------------tTPLLFDgNFNPKpAYNAIVq--nLqq---------- * 1TUX0_Thermoascus_aurantiacus -----------------------tTPLLFDgNFNPKpAYNAIVq--lL------------ * Agaricus_bisporus -----------------------sSPLLFDnNYrPKaAYNAVIN--aL------------ * Aspergillus_aculeatus -----------------------sSPLLFDsNYNPKaAYTAIaN--aL------------ * Aspergillus_flavus -----------------------eSPLLFDgNYqPKsAYNAILN--aL------------ * Aspergillus_niger -----------------------sTPLLFDsNYNPKpAYTAIaN--aL------------ * Fusarium_oxysporum -----------------------eNPLLYDsNYqPKaAFNALVN--aL------------ * Gibberella_zeae -----------------------sNPLLFDrNYqPKaAYTAVsN--aLn----------- * Neurospora_crassa -----------------------gNPLLFDaNWNPKaAYNsIVq--aLq----------- * Penicillium_chrysogenum -----------------------eSPLLFDaSYNPKeAYN-Vsq--lLsrqhafdlylkl * Trichoderma_reesei -----------------------tNPLLFDaNFNPKpAYNsIVg--iLq----------- ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS dangnvvvdpnapyakvekgkgkdaPfVFgpDYkvKpAYwAIID--hk------------ B 1R86A_Bacillus_stearothermophilus dangnvvvdpnapyakvekgkgkdaPfVFgpDYkvKpAYwAIID--hk------------ ================================================================================================ B STREPTOMYCES_COELICOLOR ------------------------TPLLFNnDgSkKaAYTAVLDalngg----------- B STREPTOMYCES_OLIVACEOVIRIDIS ------------------------TPLLFNgDgSkKaAYTAVLNalngg--ss------- B Cellulomonas_fimi ------------------------TPLLFDgSgNkKaAYTsVLNalnaggtttptpnptn B Nonomuraea_flexuosa ------------------------NPLLFDnNgNkKqAYyAVLDalneg--sddgggpsn B Streptomyces_avermitilis ------------------------TPLLFNnDgSkKqAYNAVLNalnag----dggg-gd B Streptomyces_thermoviolaceus ------------------------TPLLFNgDgSkKpAYSAVLDalngg----------- ================================================================================================ B PSEUDOMONAS_AERUGINOSA n--------------------lpdwPLLFNdNlqPKpAYqgVVE--aLsgr--------- B 1W3HB_Pseudomonas_aeruginosa n--------------------lpdwPLLFNdNlqPKpAYqgVVE--aLsgr--------- ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM ------------------------NPLIYDsNYNPKpAYNAIkEalmgy----------- ================================================================================================ Consensus xxxxxxxxxxxxxxxxxxxxxxxxSPLLFNxNFNPKxAYSAIVDxxxlxxxxxxxxxxxx 550 560 570 580 590 600 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS ------------------------------------------------------------ * PENICILLIUM_SIMPLICISSIMUM ------------------------------------------------------------ * THERMOASCUS_AURANTIACUS ------------------------------------------------------------ * 1TUX0_Thermoascus_aurantiacus ------------------------------------------------------------ * Agaricus_bisporus ------------------------------------------------------------ * Aspergillus_aculeatus ------------------------------------------------------------ * Aspergillus_flavus ------------------------------------------------------------ * Aspergillus_niger ------------------------------------------------------------ * Fusarium_oxysporum ------------------------------------------------------------ * Gibberella_zeae ------------------------------------------------------------ * Neurospora_crassa ------------------------------------------------------------ * Penicillium_chrysogenum gnlllsrlhsd------------------------------------------------- * Trichoderma_reesei ------------------------------------------------------------ ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS ------------------------------------------------------------ B 1R86A_Bacillus_stearothermophilus ------------------------------------------------------------ ================================================================================================ B STREPTOMYCES_COELICOLOR ------------------------------------------------------------ B STREPTOMYCES_OLIVACEOVIRIDIS ---tppp--s------------------gg-gqikgvg---------------------- B Cellulomonas_fimi p----tptptptn-pgggspsctatysegqkwgdrfngt-vtirattnisswqstvtvrs B Nonomuraea_flexuosa ppvspppgggsgqirgvasnrc-----idvpngntadgtqvqlydchsgsnqqwtytssg B Streptomyces_avermitilis pd--pepgdgta-ikgvgsgrc-----ldvpnasttdgtqvqlydcngrtnqqweltssg B Streptomyces_thermoviolaceus ssgeppedggsgqiknaasgrc-----ldvsgagtadgtavqlydchggtnqqwtytdag ================================================================================================ B PSEUDOMONAS_AERUGINOSA ------------------------------------------------------------ B 1W3HB_Pseudomonas_aeruginosa ------------------------------------------------------------ ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM ------------------------------------------------------------ ================================================================================================ Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 610 620 630 640 650 660 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx ================================================================================================ * EMERICELLA_NIDULANS ------------------------------------------------------------ * PENICILLIUM_SIMPLICISSIMUM ------------------------------------------------------------ * THERMOASCUS_AURANTIACUS ------------------------------------------------------------ * 1TUX0_Thermoascus_aurantiacus ------------------------------------------------------------ * Agaricus_bisporus ------------------------------------------------------------ * Aspergillus_aculeatus ------------------------------------------------------------ * Aspergillus_flavus ------------------------------------------------------------ * Aspergillus_niger ------------------------------------------------------------ * Fusarium_oxysporum ------------------------------------------------------------ * Gibberella_zeae ------------------------------------------------------------ * Neurospora_crassa ------------------------------------------------------------ * Penicillium_chrysogenum ------------------------------------------------------------ * Trichoderma_reesei ------------------------------------------------------------ ================================================================================================ B BACILLUS_STEAROTHERMOPHILUS ------------------------------------------------------------ B 1R86A_Bacillus_stearothermophilus ------------------------------------------------------------ ================================================================================================ B STREPTOMYCES_COELICOLOR ------------------------------------------------------------ B STREPTOMYCES_OLIVACEOVIRIDIS ------------------------------------------------------------ B Cellulomonas_fimi pqkii-a------------------twng-sptwdssgnvmtmrpsgsgal-aagqstsf B Nonomuraea_flexuosa efrifgnkcldaggssngavvqiyscwgganqkwelradgtivgvqsglcldavgggtgn B Streptomyces_avermitilis elrvygnkcldaagtgngakvqiyscwggdnr------sgv------------------- B Streptomyces_thermoviolaceus efrvygnkcldaggtgngarvqiyscwggdnqkwrvnsdgtivgvqsglclda--agtgn ================================================================================================ B PSEUDOMONAS_AERUGINOSA ------------------------------------------------------------ B 1W3HB_Pseudomonas_aeruginosa ------------------------------------------------------------ ================================================================================================ B CLOSTRIDIUM_THERMOCELLUM ------------------------------------------------------------ ================================================================================================ Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 670 680 690 700 710 720 ----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxx ======================================================= * EMERICELLA_NIDULANS ------------------- * PENICILLIUM_SIMPLICISSIMUM ------------------- * THERMOASCUS_AURANTIACUS ------------------- * 1TUX0_Thermoascus_aurantiacus ------------------- * Agaricus_bisporus ------------------- * Aspergillus_aculeatus ------------------- * Aspergillus_flavus ------------------- * Aspergillus_niger ------------------- * Fusarium_oxysporum ------------------- * Gibberella_zeae ------------------- * Neurospora_crassa ------------------- * Penicillium_chrysogenum ------------------- * Trichoderma_reesei ------------------- ======================================================= B BACILLUS_STEAROTHERMOPHILUS ------------------- B 1R86A_Bacillus_stearothermophilus ------------------- ======================================================= B STREPTOMYCES_COELICOLOR ------------------- B STREPTOMYCES_OLIVACEOVIRIDIS ------------------- B Cellulomonas_fimi gftvqhngnwtwpsvtcaa B Nonomuraea_flexuosa gtrlqlyscwggnnqkwsy B Streptomyces_avermitilis ------------------- B Streptomyces_thermoviolaceus stpvqlytcsyadnqrwtv ======================================================= B PSEUDOMONAS_AERUGINOSA ------------------- B 1W3HB_Pseudomonas_aeruginosa ------------------- ======================================================= B CLOSTRIDIUM_THERMOCELLUM ------------------- ======================================================= Consensus xxxxxxxxxxxxxxxxxxx

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