Cellulase B (glycosyl hydrolase 6; 1,4-beta-cellobiohydrolase)

10 20 30 40 50 60 70 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----: Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxixxxxxxixxxxxsxxxxxxqxxxxxxxxxxxxxxxxxxxxxxx ==================================================================================================== HUMICOLA_INSOLENS --akffltaafaaaalaapv-veerqnca-ptwgqcggIgfngpTccqsgstcvkqndwysqclpg-sqvtttst 1GZ1A_Humicola_insolens --------------------------------------------------------------------------- 1OCNA_Humicola_insolens --------------------------------------------------------------------------- Chaetomium_thermophilum a-kqllltaalaaislaapl-leerqscs-sVwgqcggInyngpTccqsgsvcaylndwysqcipg-qaqpgtts Fusarium_oxysporum --ayklilaafaatalaap--veerqscsngVwaqcggqnwsgtpcctsgnKcvklndfysqcqpg-s----aep Gibberella_zeae tayklflaaafaatalaap--veerqscsngVwsqcggqnwsgtpcctsgnKcvkvndfysqcqpg-s--adpsp Magnaporthe_grisea a-sklflaaallqgalssplaveerqaca-aqwgqcggqdytgpTccqsgstcvvsnqwysqclpgssnptttsr Neurospora_crassa aakklllaaaltasalaapv-ledrqncg-sawsqcggIgwsgaTccssgnscveinsyysqclpg-aqvtttag ==================================================================================================== TRICHODERMA_REESEI ----------------------------ivgIlttlatLatlaaSvpleerQacssvwgqcggqnwsgptccasg 1HGWA_Trichoderma_reesei --------------------------------------------------------------------------- 1HGYA_Trichoderma_reesei --------------------------------------------------------------------------- Emericella_nidulans -----------------------------------hysasglalafllpaiQaqqtlygqcggsgwtgatscvag Hypocrea_koningii ----------------------------ivgIlttlatLatlaaSvpleerQacssvwgqcggqnwsgptccasg Talaromyces_emersonii ---------------------------------rnllaLapaallvgaaeaQ--qslwgqcggsswtgatscaag Trichoderma_parceramosum ----------------------------ivgIlttlatLatlaaSvpleerQacssvwgqcggqnwsgptccaag Trichoderma_viride ----------------------------ivgIlttlatLatlaaSvpleerQacssvwgqcggqnwsgptccasg ==================================================================================================== Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxixxxxxxixxxxxsxxxxxxqxxxxxxxxxxxxxxxxxxxxxxx 80 90 100 110 120 130 140 150 ----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus ssxxxxxxxxxxxxxxsxxtsxxtxxxxxxxxxxxtxxxssxtsxpxxtttxxxxxxxtxtXxysGNPFxGVxLW ==================================================================================================== HUMICOLA_INSOLENS TStss-------ssttSraTS----------ttr-TggvTSiTTaPtrTvTipggattTap--YNGNPFeGVqLW 1GZ1A_Humicola_insolens ---------------------------------------------------------------YaGNPFeGVqLW 1OCNA_Humicola_insolens -----------------------------------------------------------------GNPFeGVqLW Chaetomium_thermophilum TTart-------tstsTtsTS----------svrpTtsnTpvTTaPp-TTTipggassTaS--YNGNPFsGVqLW Fusarium_oxysporum SStaa-------gpssTtaTk----------tta-Tgg--SsTTaggsv--------tSappaaSdNPYaGVdLW Gibberella_zeae TStiv-------sattTkaTT----------tgs-ggsvT---SpP------p--vat-------nNPFsGVdLW Magnaporthe_grisea TStss-------ssstSrtSSstSrppssvpttp-TsvppTiTTtPttTpTggsgpgtTaS--FTGNPFaGVnLF Neurospora_crassa aSstsptstskvssttSkvTS----------ssa-aqpiTTtTapsvpTTTiaggassTaS--FTGNPFlGVqgW ==================================================================================================== TRICHODERMA_REESEI STcvysndyysqclpgaasSSssTraasttsrvspTtsrSSsaTpPpgSTTtrvppvgSgTatYSGNPFvGVtpW 1HGWA_Trichoderma_reesei ------------------------------------------------------------TatYSGNPFvGVtpW 1HGYA_Trichoderma_reesei ------------------------------------------------------------TatYSGNPFvGVtpW Emericella_nidulans aacstlnqwyaqclp--aaTTtsTtltttt--------sSvtTTsnpgSTTt-ts-svTvTataSGNPFsGyqLY Hypocrea_koningii STcvysndyysqclpgaasSSssTraasttsrvspTtsrSSsaTpPpgSTTtrvppvgSgTatYSGNPFvGVtpW Talaromyces_emersonii aTcstinpyyaqcvpaTatpTtlTtttkpts-----tggaapTTpPptTTgtttspvvTrpasaSGNPFeGyqLY Trichoderma_parceramosum STcvysndyysqcppgaasSSssTrassttnrvssTts-TSsaTpPpgSTTtrvppvgSgTatYSGNPFvGVtpW Trichoderma_viride STcvysndyysqclpgaasSSssTraasttsrvspTtsrSSsaTpPpgSTTtrvppvgSgTatYSGNPFvGVtpW ==================================================================================================== Consensus ssxxxxxxxxxxxxxxsxxtsxxtxxxxxxxxxxxtxxxssxtsxpxxtttxxxxxxxtxtXxysGNPFxGVxLW 160 170 180 190 200 210 220 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----: Consensus ANxYYxSEVxSLAIPSITxxALASAAAAVAEVPSFQWLDxxxKVXXLLEZSLAEIRxANQAGxxxXxxYAGQFVV ==================================================================================================== HUMICOLA_INSOLENS ANnYYrSEVhTLAIPqITdpALraAAsAVAEVPSFQWLDrnvtVdtLLvZTLsEIReANQAGanpq--YAaQiVV 1GZ1A_Humicola_insolens ANnYYrSEVhTLAIPqITdpALraAAsAVAEVPSFQWLDrnvtVdtLLvQTLsEIReANQAGanpq--YAaQiVV 1OCNA_Humicola_insolens ANnYYrSEVhTLAIPqITdpALraAAsAVAEVPSFQWLDrnvtVdtLLvQTLsEIReANQAGanpq--YAaQiVV Chaetomium_thermophilum ANtYYsSEVhTLAIPSLS-peLAakAAkVAEVPSFQWLDrnvtVdtLfsgTLAEIRaANQrGanpp--YAGiFVV Fusarium_oxysporum ANnYYrSEVmNLAVPkLSg-AkATAAAkVADVPSFQWMDtydhI-sLMEDTLADIRkANKAGgk----YAGQFVV Gibberella_zeae ANnYYrSEVsTLAIPkLSg-AMATAAAkVADVPSFQWMDtydhI-sfMEESLADIRkANKAGgn----YAGQFVV Magnaporthe_grisea pNkFYsSEVhTLAIPSLTg-sLvakAsAVAQVPSFQWLDiaaKVetLMpgaLADVRaANaAGgn----YAaQlVV Neurospora_crassa ANsYYsSEIyNhAIPSMTg-sLAaqAsAVAKVPTFQWLDrnvtVdtLMKsTLeEIRaANKAGanpp--YAahFVV ==================================================================================================== TRICHODERMA_REESEI ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV 1HGWA_Trichoderma_reesei ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV 1HGYA_Trichoderma_reesei ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV Emericella_nidulans vNpYYsSEVqSIAIPSLT-gtLsSlApAataaaktR--DvaaKVp-tMatyLADIRsqNaAG--anppiAGQFVV Hypocrea_koningii ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV Talaromyces_emersonii ANpYYaSEViSLAIPSLS-seLvpkAseVAKVPSFvWLDqaaKVp-sMgDyLkDIQsqNaAG--adppiAGiFVV Trichoderma_parceramosum ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV Trichoderma_viride ANaYYaSEVsSLAIPSLT-gAMATAAAAVAKVPSFmWLDtldKtp-LMEQTLADIRtANKnG--gn--YAGQFVV ==================================================================================================== Consensus ANxYYxSEVxSLAIPSITxxALASAAAAVAEVPSFQWLDxxxKVXXLLEZSLAEIRxANQAGxxxXxxYAGQFVV 230 240 250 260 270 280 290 300 ----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus YDLPDRDCAAAASNGEWSIANxGVxKYKxYINxIREILIEFSDVRTILVIEPDSLANMVTNMNVPKCANAQSAYL ==================================================================================================== HUMICOLA_INSOLENS YDLPDRDCAAAASNGEWaIANnGVnnYKaYINrIREILIsFSDVRTILVIEPDSLANMVTNMNVPKCsgAaStYr 1GZ1A_Humicola_insolens YDLPDRDCAAAASNGEWaIANnGVnnYKaYINrIREILIsFSDVRTILVIEPDSLANMVTNMNVPKCsgAaStYr 1OCNA_Humicola_insolens YDLPDRDCAAAASNGEWaIANnGVnnYKaYINrIREILIsFSDVRTILVIEPDSLANMVTNMNVPKCsgAaStYr Chaetomium_thermophilum YDLPDRDCAAAASNGEWSIANnGannlQrYIDrIRELLIQYSDIRTILVIEPDSLANMVTNMNVqKCsNAaStYk Fusarium_oxysporum YDLPNRDCAAAASNGEYSLdkdGanKYKaYIakIKgILqnYSDtKvILVIEPDSLANLVTNLNVdKCAkAESAYk Gibberella_zeae YDLPDRDCAAAASNGEYSLdkdGknKYKaYIakIKgILqDYSDtRiILVIEPDSLANMVTNMNVPKCANAaSAYk Magnaporthe_grisea YDLPDRDCAAAASNGEFSIADgGVvKYKaYIDaIRKqLLaYSDVRTILVIEPDSLANMVTNMgVPKCAgAKdAYL Neurospora_crassa YDLPDRDCAAAASNGEFSIANgGVanYKtYINaIRKLLIEYSDIRTILVIEPDSLANLVTNtNVaKCANAaSAYr ==================================================================================================== TRICHODERMA_REESEI FDLPDRDCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTLLVIEPDSLANLVTNLgtPKCANAQSAYL 1HGWA_Trichoderma_reesei YDLPDRaCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTLLVIEPDSLANLVTNLgtPKCANAQSAYL 1HGYA_Trichoderma_reesei YDLPDRDCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTLLVIEPaSLANLVTNLgtPKCANAQSAYL Emericella_nidulans YDLPDRDCAAlASNGEFaIsDgGVqhYKdYIDsIREILVEYSDVhvILVIEPDSLANLVTNLNVaKCANAQSAYL Hypocrea_koningii YDLPDRDCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTLLVIEPDSLANLVTNLgtPKCANAQSAYL Talaromyces_emersonii YDLPDRDCAAAASNGEFSIANnGValYKqYIDsIREqLttYSDVhTILVIEPDSLANVVTNLNVPKCANAQdAYL Trichoderma_parceramosum YDLPDRDCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTILVIEPDSLANLVTNLgtPKCANAQSAYL Trichoderma_viride YDLPDRDCAAlASNGEYSIADgGVaKYKnYIDtIRQIVVEYSDIRTLLVIEPDSLANLVTNLgtPKCANApSAYL ==================================================================================================== Consensus YDLPDRDCAAAASNGEWSIANxGVxKYKxYINxIREILIEFSDVRTILVIEPDSLANMVTNMNVPKCANAQSAYL 310 320 330 340 350 360 370 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----: Consensus ECTNYALTQLDLPHVAMYMDAGHAGWLGWPANIxPAAELFAXIYKDAGXPxAVRGLATNVANYNxWSVSSXPSYT ==================================================================================================== HUMICOLA_INSOLENS ElTiYALkQLDLPHVAMYMDAGHAGWLGWPANIqPAAELFAkIYEDAGkPrAVRGLATNVANYNaWSVSSpPpYT 1GZ1A_Humicola_insolens ElTiYALkQLDLPHVAMYMDAGHAGWLGWPANIqPAAELFAkIYEDAGkPrAVRGLATNVANYNaWSVSSpPpYT 1OCNA_Humicola_insolens ElTiYALkQLDLPHVAMYMDAGHAGWLGWPANIqPAAELFAkIYEDAGkPrAVRGLATNVANYNaWSVSSpPpYT Chaetomium_thermophilum ElTvYALkQLNLPHVAMYMDAGHAGWLGWPANIqPAAELFAqIYRDAGrPaAVRGLATNVANYNaWSIaSpPSYT Fusarium_oxysporum ElTvYAIkELNLPNVsMYLDAGHgGWLGWPANIgPAAKLYAqIYKDAGkPsrVRGLvTNVsNYNgWkLSTkPdYT Gibberella_zeae ElTihALkELNLPNVsMYIDAGHgGWLGWPANLpPAAQLYgqLYKDAGkPsrLRGLvTNVsNYNaWkLSSkPdYT Magnaporthe_grisea ECTiYAVkQLNLPHVAMYLDgGHAGWLGWPANLqPAADLFgkLYaDAGkPsqLRGMATNVANYNaWdLTTaPSYT Neurospora_crassa ECTNYAITQLDLPHVAqYLDAGHgGWLGWPANIqPAAtLFAdIYKaAGkPksVRGLvTNVsNYNgWSLSSaPSYT ==================================================================================================== TRICHODERMA_REESEI ECiNYAVTQLNLPNVAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPrALRGLATNVANYNgWNITSpPSYT 1HGWA_Trichoderma_reesei ECiNYAVTQLNLPNVAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPrALRGLATNVANYNgWNITSpPSYT 1HGYA_Trichoderma_reesei ECiNYAVTQLNLPNVAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPrALRGLATNVANYNgWNITSpPSYT Emericella_nidulans ECTNYAVTQLNLPNVAMYLDAGHAGWLGWPANLqPAAnLYAgVYsDAGsPaALRGLATNVANYNaWaIdTcPSYT Hypocrea_koningii ECiNYAVTQLNLPNVAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPrALRGLATNVANYNgWNITSpPSYT Talaromyces_emersonii ECiNYAITQLDLPNVAMYLDAGHAGWLGWqANLaPAAQLFAsVYKNAssPasVRGLATNVANYNaWSISrcPSYT Trichoderma_parceramosum ECiNYAITQLNLPNIAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPsALRGLATNVANYNgWNITSpPSYT Trichoderma_viride ECiNYAVTQLNLPNVAMYLDAGHAGWLGWPANqdPAAQLFAnVYKNAssPrALRGLATNVANYNgWNITSpPSYT ==================================================================================================== Consensus ECTNYALTQLDLPHVAMYMDAGHAGWLGWPANIxPAAELFAXIYKDAGXPxAVRGLATNVANYNxWSVSSXPSYT 380 390 400 410 420 430 440 450 ----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus QXNXNYDEKxYINAIxPLLxxQGFxxAxFIVDQGRSGKQPTGQKEWGDWCNVIGTGFGMRPTxNTGNxLVDAFVW ==================================================================================================== HUMICOLA_INSOLENS spNpNYDEKhYIeAfrPLLeaRGFp-AqFIVDQGRSGKQPTGQKEWGhWCNaIGTGFGMRPTaNTGHqyVDAFVW 1GZ1A_Humicola_insolens spNpNYDEKhYIeAfrPLLeaRGFp-AqFIVDQGRSGKQPTGQKEWGhWCNaIGTGFGMRPTaNTGHqyVDAFVW 1OCNA_Humicola_insolens spNpNYDEKhYIeAfrPLLeaRGFp-AqFIVDQGRSGKQPTGQKEWGhWCNaIGTGFGMRPTaNTGHqyVDAFVW Chaetomium_thermophilum spNpNYDEKhYIeAfaPLLrnQGFd-AkFIVDtGRNGKQPTGQlEWGhWCNVkGTGFGVRPTaNTGHeLVDAFVW Fusarium_oxysporum EsNpNYDEQrYINAfaPLLaqEGWsnvkFIVDQGRSGKQPTGQKaqGDWCNakGTGFGLRPStNTGDaLaDAFVW Gibberella_zeae EsNpNYDEQkYIHALsPLLeqEGWpgAkFIVDQGRSGKQPTGQKaWGDWCNapGTGFGLRPSaNTGDaLVDAFVW Magnaporthe_grisea tpNpNFDEKkYISAfaPLLaaKGWs-AhFIIDQGRSGKQPTGQKEWGhWCNqqGvGFGrRPSaNTGSeLaDAFVW Neurospora_crassa tpNpNYDEKkYIeAfsPLLnaaGFp-AqFIVDtGRSGKQPTGQiEqGDWCNaIGTGFGVRPTtNTGSsLaDAFVW ==================================================================================================== TRICHODERMA_REESEI QgNavYNEKlYIHAIgPLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSaNTGDsLLDsFVW 1HGWA_Trichoderma_reesei QgNavYNEKlYIHAIgPLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSaNTGDsLLDsFVW 1HGYA_Trichoderma_reesei QgNavYNEKlYIHAIgPLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSaNTGDsLLDsFVW Emericella_nidulans QgNsvcDEKdYINALaPLLraQGF-dAhFItDtGRNGKQPTGQQaWGDWCNVIGTGFGaRPStNTGDsLLDAFVW Hypocrea_koningii QgNavYNEKlYIHAIgrLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSaNTGDsLLDsFVW Talaromyces_emersonii QgDaNcDEEdYVNALgPLfqeQGFp-AyFIIDtsRNGvRPTkQsQWGDWCNVIGTGFGVRPTtDTGNpLeDAFVW Trichoderma_parceramosum QgNavYNEKlYIHAIgPLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSsNTGDsLLDsFVW Trichoderma_viride QgNavYNEKlYIHAIgPLLanhGWsnAfFItDQGRSGKQPTGQQQWGDWCNVIGTGFGIRPSaNTGDsLLDsFVW ==================================================================================================== Consensus QXNXNYDEKxYINAIxPLLxxQGFxxAxFIVDQGRSGKQPTGQKEWGDWCNVIGTGFGMRPTxNTGNxLVDAFVW 460 470 480 490 500 510 520 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----: Consensus VKPGGEXDGTSDTTAxRYDXHCGLxxDALKPAPEAGxWFQAYFXQLLxNANPSFx ================================================================================ HUMICOLA_INSOLENS VKPGGEcDGTSDTTAaRYDyHCGLe-DALKPAPEAGqWFneYFiQLLrNANPpF- 1GZ1A_Humicola_insolens VKPGGEcDGTSDTTAaRYayHCGLe-DALKPAPEAGqWFneYFiQLLrNANPpF- 1OCNA_Humicola_insolens VKPGGEcDGTSDTTAaRYayHCGLe-DALKPAPEAGqWFneYFiQLLrNANPpF- Chaetomium_thermophilum VKPGGEsDGTSDTSAaRYDyHCGLs-DALtPAPEAGqWFQAYFeQLLiNANPpF- Fusarium_oxysporum VKPGGEsDGTSDTSAaRYDyHCGLd-DALKPAPEAGtWFQAYFeQLLdNANPSFl Gibberella_zeae VKPGGEsDGTSDTSAaRYDyHCGId-gAVKPAPEAGtWFQAYFeQLLkNANPSFl Magnaporthe_grisea IKPGGEcDGvSDpTApRFDhfCGtdygAMsdAPQAGqWFQkYFemLLtNANPpl- Neurospora_crassa VKPGGEsDGTSDTSAtRYDyHCGLs-DALKPAPEAGqWFQAYFeQLLkNANPaF- ================================================================================ TRICHODERMA_REESEI VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl 1HGWA_Trichoderma_reesei VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl 1HGYA_Trichoderma_reesei VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl Emericella_nidulans VKPGGEsDGTSDTSAaRYDaHCGys-DALQPAPEAGtWFQAYFvQLLqNANPSF- Hypocrea_koningii VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl Talaromyces_emersonii VKPGGEsDGTSNTTspRYDyHCGLs-DALQPAPEAGtWFQAYFeQLLtNANPlF- Trichoderma_parceramosum VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl Trichoderma_viride VKPGGEcDGTSDSSApRFDsHCaLp-DALQPAPQAGaWFQAYFvQLLtNANPSFl ================================================================================ Consensus VKPGGEXDGTSDTTAxRYDXHCGLxxDALKPAPEAGxWFQAYFXQLLxNANPSFx