10        20        30        40        50        60        70
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     xxxxxxxxnxnxnxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxpRVYksIVzdVVnEVREiFlN
====================================================================================================
YEAST                         -----------------------------snaea---------------sRVYEiIVZsVVNEVREdFeN
Saccharomyces_cerevisiae-TOA1 -----------------------------snaea---------------sRVYEiIVEsVVNEVREdFeN
====================================================================================================
HUMAN                         ------anSaNtN-------------------tv---------------PKLYRSVIEDVINDVRDIFLD
Canis_lupus                   rycwafkeNdNrN-------------------tasyinselqvfifplkPKLYRSVIEDVINDVRDIFLD
Danio_rerio                   ------asSaNsN-------------------qv---------------PKLYRSVMEDVIaEVRELFLD
Gallus_gallus                 ----------------------------------------------------------------------
Mus_musculus                  ------anSaNtN-------------------tv---------------PKLYRSVIEDVINDVRDIFLD
Pan_troglodytes               pivsstsrStktNkkslqwskcprfrleiylllp---------------PKLYRSVIEDVINDVRDIFLD
Pongo_pygmaeus                ------anSaNtN-------------------tv---------------PKLYRSVIEDVINDVRDIFLD
Rattus_norvegicus             ------anSaNtN-------------------tv---------------PKLYRSVIEDVINDVRDIFLD
Tetraodon_nigroviridis        -------------------------------------------------PKVYKSVIDDVINEVRELFLD
Xenopus_laevis                ---as-aaSaNaN-------------------pv---------------PKLYRSVIgDVINDVREIFLD
Xenopus_tropicalis            ------aaSaNaN-------------------pv---------------PKLYRSVIEDVINDVRELFLD
====================================================================================================
Consensus                     xxxxxxxxnxnxnxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxpRVYksIVzdVVnEVREiFlN

                                      80        90       100       110       120       130       140
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     dGIdEQvLmDLKtIxxxxxxxxxxxxxxWQnxxxxxKLmexxxtravdgxxxxxxxxxfhsexeqqlllq
====================================================================================================
YEAST                         aGIDEQtLqDLKnI--------------WQk-----KLtEtkvTtfswdnqfnegningvqndlnfnLat
Saccharomyces_cerevisiae-TOA1 aGIDEQtLqDLKnI--------------WQk-----KLtEtkvTtfswdnqfnegningvqndlnfnLat
====================================================================================================
HUMAN                         DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Canis_lupus                   DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Danio_rerio                   EGVDEQVLMELKTL--------------WES-----KLMQ---SKAVDG---------FHTE-EQQVL--
Gallus_gallus                 ---------ELKTL--------------WEN-----KLMQ---SKAVDG---------FHSE-EQQLLLQ
Mus_musculus                  DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Pan_troglodytes               DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Pongo_pygmaeus                DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Rattus_norvegicus             DGVDEQVLMELKTL--------------WEN-----KLMQ---SRAVDG---------FHSE-EQQLLLQ
Tetraodon_nigroviridis        EGVDEQVLLELKTVhnstvsfsvnihytWENlhrhtpLfsqegSKpLNkhfpvcsnflFyvKysppfLfQ
Xenopus_laevis                EGVgEQVLMELKTL--------------WES-----KLMQ---SKAVDG---------FHTE-EQQLLLQ
Xenopus_tropicalis            EGVDEQVLMELKTL--------------WES-----KLMQ---SKAVDG---------FHSE-EQQLLLQ
====================================================================================================
Consensus                     dGIdEQvLmDLKtIxxxxxxxxxxxxxxWQnxxxxxKLmexxxtravdgxxxxxxxxxfhsexeqqlllq

                                     150       160       170       180       190       200       210
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     vxqqqxxxxxxxxxxxxxqxqqqxxnhnnnxxqqxepqqtvxqqxqtqqvlipasqqaxxxxxqvvvxes
====================================================================================================
YEAST                         pgvnssefnikeentgneglilpniNsNNNiphsgEtnintntveaTnnsgatlNtntsgntnadVtsQp
Saccharomyces_cerevisiae-TOA1 pgvnssefnikeentgneglilpniNsNNNiphsgEtnintntveaTnnsgatlNtntsgntnadVtsQp
====================================================================================================
HUMAN                         V-QQQh------------QpQQQqhHHHHH-hQQaQPQQTVpQQaQTQQVLIPASQQAtap--QVIVpDS
Canis_lupus                   V-QQQh------------QpQQQqhHHHHH-hQQtQPQQTVpQQaQTQQVLIPASQQAaap--QVIVpDS
Danio_rerio                   ---------------------------Haq-qQQaQaQQT-qQQtQpQQVLLPpaQttpqq--QVIVqDa
Gallus_gallus                 VqQQQq------------QqQQQqhHHHHH-htQpQPQQTVqQQtQpQQVLIPASQQApqq--QVIVpDS
Mus_musculus                  V-QQQh------------QpQQQqhHHHHHqhQQaQPQQTVpQQaQTQQVLIPASQQAtap--QVIVpDS
Pan_troglodytes               V-QQQh------------QpQQQqhHHHHH-hQQaQPQQTVpQQaQTQQVLIPASQQAtap--QVIVpDS
Pongo_pygmaeus                V-QQQh------------QpQQQqhHHHHH-hQQaQPQQTVpQQaQTQQVLIPASQQAtap--QVIVpDS
Rattus_norvegicus             V-QQQh------------QpQQQqhHHHHH-hQQaQPQQTVpQQaQTQQVLIPASQQAtap--QVIVpDS
Tetraodon_nigroviridis        L-wEnkllqskavegfytEeQtalqaaqqq-qhQvQPvQqVaQptQaQQVILPpqpQqqapqqQVIVqDS
Xenopus_laevis                a-QQQq------------QqQQQhsqHHrv---QqhPQQqqaashQTQQVLIPAThQAqqq--QVLVqES
Xenopus_tropicalis            a-QQQq------------hpQ-----HHHv-sQhpQqQQaashQtQTQQVLIPAThQApqq--QVLVqDS
====================================================================================================
Consensus                     vxqqqxxxxxxxxxxxxxqxqqqxxnhnnnxxqqxepqqtvxqqxqtqqvlipasqqaxxxxxqvvvxes

                                     220       230       240       250       260       270       280
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     kiiqhmsasxmxnaaataatlaipagvtpvqqiltnsgqllqvvraangaqyilxqpkesvvlqqqvipq
====================================================================================================
YEAST                         KIevkpeieltiNnAnittvenIddesekkddeekeedvektrkekeqieQvkLqakKEkrsalldtdev
Saccharomyces_cerevisiae-TOA1 KIevkpeieltiNnAnittvenIddesekkddeekeedvektrkekeqieQvkLqakKEkrsalldtdev
====================================================================================================
HUMAN                         KLIQHMNASnM-SAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIf-QPQQSVVLQQQVIPQ
Canis_lupus                   KLIQHMNASnM-SAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIf-QPQQSVVLQQQVIPQ
Danio_rerio                   KIMQHMSATgM-SAAATAATLALPtGVaPfQQLITpqGQILQVVRAANGAQYII-QPQQqMLLQQQVLPQ
Gallus_gallus                 KLIpHMNASgM-SAAATvATLALPAGVTPVQQILTNSGQILQVVRtANGAQYII-QPQQpVVLQQQVIPQ
Mus_musculus                  KLLQHMNASsItSAAATAATLALPAGVTPVQQLLTNSGQLLQVVRAANGAQYIL-QPQQSVVLQQQVIPQ
Pan_troglodytes               KLIQHMNASnM-SAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIf-QPQQSVVLQQQVIPQ
Pongo_pygmaeus                KLIQHMNASnM-SAAATAATLALPAGVTPVQQILTNSGQLLQVVRvANGAQYIf-QPQQSVVLQQQVIPQ
Rattus_norvegicus             KLIQHMNASsItSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIf-QPQQSVVLQQQVIPQ
Tetraodon_nigroviridis        KILQHMSATgM-SAAATAATLALPAGVTPyQQLLTSqGQLLQVVRAANGtQYLIqQPQQqILLQQ----Q
Xenopus_laevis                KMIhHMTpqgM-SA---AtTLALPAGVTPVQQLITNSGQLLQVVRAANGAQYLI-QPQQSMVLQQQVIPQ
Xenopus_tropicalis            KLIQHMTtqgM-SAAATAATLALPAGVTPVQQLITNSGQLLQVVRAANGAQYLI-QPQQSMVLQQQVIPQ
====================================================================================================
Consensus                     kiiqhmsasxmxnaaataatlaipagvtpvqqiltnsgqllqvvraangaqyilxqpkesvvlqqqvipq

                                     290       300       310       320       330       340       350
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     mqpggvqapviqqvlaplpggisxqtgviiqpqqilxftgnkxxxxtqvipttvxaxxxxpxxpaqaqix
====================================================================================================
YEAST                         gselddsdddylisegeedG--------------------------------------------------
Saccharomyces_cerevisiae-TOA1 gselddsdddylisegeedG--------------------------------------------------
====================================================================================================
HUMAN                         MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Canis_lupus                   MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Danio_rerio                   MQPGGVQAPVIQQVLtPLqGGLpqQTGVIIQPQQIV-lTGNKvpqnTQVMqaaamAvqsgP-gdAQvQtq
Gallus_gallus                 MQPGGVQAPVIQQVLAPIPGGIpqQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Mus_musculus                  MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-aPAQApM-
Pan_troglodytes               MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Pongo_pygmaeus                MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Rattus_norvegicus             MQPGGVQAPVIQQVLAPLPGGISpQTGVIIQPQQIL-FTGNK----TQVIPTTVaA----P-tPAQAQI-
Tetraodon_nigroviridis        MQPaGVQAPVIQQVLtPLPGaLpqQTGVIIQPQQIVlaSGNK----vQg-nTqVgt----PlcPsyApp-
Xenopus_laevis                MQqGGVQAPVIQQVLtPMPGGLSqQTGVIIQPQQIL-FTGNK----TQVIPnTMsA----PqaPiQgQL-
Xenopus_tropicalis            MQPGGVQAPVIQQVLAPIPGGLSqQTGVIIQPQQIL-FTGNK----TQVIPTTMsA----PqaPtQgQL-
====================================================================================================
Consensus                     mqpggvqapviqqvlaplpggisxqtgviiqpqqilxftgnkxxxxtqvipttvxaxxxxpxxpaqaqix

                                     360       370       380       390       400       410       420
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     xxaxxqqqxqxqxaqxqaplvlqvdgxgdtsseededeeedyddeeeedkekdgxedgqveeeplnsedd
====================================================================================================
YEAST                         ----------------------------------------------------------------------
Saccharomyces_cerevisiae-TOA1 ----------------------------------------------------------------------
====================================================================================================
HUMAN                         -tAtgQQQpQaQpAQtQAPLVLQVDGtGDTSSEEDEDEEEDYDDDEEEDKEKDGaEDGQVEEEPLNSEDD
Canis_lupus                   -tAtgQQQpQtQpAQpQAPLVLQVDGtGDTSSEEDEDEEEDYDDEEEEDKEKDGaEDGQVEEEPLNSEDD
Danio_rerio                   pqAqqQQQqQqQqAsqQpPMMLQVDGaGDTSSEEDEEEEDEYDEDEDEDKEKDGgEDGQVEEEPLNSgDD
Gallus_gallus                 -pAagQQQpQqQqAQpQAPLVLQVDGaGDTSSEEEEDEEEDYDDDEEEDKEKDGgEDGQVEEEPLNSEDD
Mus_musculus                  -pAagQQQpQaQpAQqQAPLVLQVDGtGDTSSEEDEDEEEDYDDDEEEDKEKDGaEDGQVEEEPLNSEDD
Pan_troglodytes               -tttgQQQpQaQpAQtQAPLVLQVDGtGDTSSEEDEDEEEDYDDDEEEDKEKDGaEDGQVEEEPLNSEDD
Pongo_pygmaeus                -tAtghQQpQaQpAQtQAPLVLQVDGtGDTSSEEDEDEEEDYDDDEEEDKEKDGaEDGQVEEEPLNSEDD
Rattus_norvegicus             -pAagQQQpQaQpAQqQAPLVLQVDGtGDTSSEEDEDEEEDYDDDEEEDKEKDGaEDGQVEEEPLNSEDD
Tetraodon_nigroviridis        -rAasvpKaQpQpqvqQpPLMLQVDGaGETSSEEEEDEEEEYDEDEDEEKEKEagEDwQVEEEPLNSgDD
Xenopus_laevis                -tvtsQQQqQ-g--QqQAPLVLQVDGaGDTSSEEDEDEDEDYDDEEEEDKEKDG-EDGQVEEEPLNSEDD
Xenopus_tropicalis            -tvtsQQQqQgQ----QqqVVLQVDGaGDTSSEEDEDEDEDYDDEEEEDKEKDG-EDGQVEEEPLNSEDD
====================================================================================================
Consensus                     xxaxxqqqxqxqxaqxqaplvlqvdgxgdtsseededeeedyddeeeedkekdgxedgqveeeplnsedd

                                     430       440       450       460       470       480       490
                              ----:----|----:----|----:----|----:----|----:----|----:----|----:----|
Consensus                     vsdeegqelfdtENLMLCqYDKVhRTKnRWKfhLKDGVVnINgrDYiFsKAigEAEWx
========================================================================================
YEAST                         ----------pdENLMLClYDKVtRTKaRWKcsLKDGVVtINrnDYtFqKAqvEAEWv
Saccharomyces_cerevisiae-TOA1 ----------pdENLMLClYDKVtRTKaRWKcsLKDGVVtINrnDYtFqKAqvEAEWv
========================================================================================
HUMAN                         VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Canis_lupus                   VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Danio_rerio                   VSDEEdQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDFVFSKAIGDAEW-
Gallus_gallus                 VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYVFSKAIGDAEW-
Mus_musculus                  VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Pan_troglodytes               VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Pongo_pygmaeus                VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Rattus_norvegicus             VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW-
Tetraodon_nigroviridis        VSDEEdQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGKDYVFSKAIGDAEW-
Xenopus_laevis                VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDFVFSKAIGDAEW-
Xenopus_tropicalis            VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDFVFSKAIGDAEW-
========================================================================================
Consensus                     vsdeegqelfdtENLMLCqYDKVhRTKnRWKfhLKDGVVnINgrDYiFsKAigEAEWx