Copy #: 3	Name:
Restriction Mapping Question: 10 Points Total

A DNA insert has been cloned in a vector, creating a plasmid. The vector is
4000 bp. At one end of the insert is a site for the
restriction enzyme AbcI (which will not cut anywhere else in the plasmid);
at the other end of the insert is a site for the restriction enzyme XyzI
(which, again, will not cut anywhere else in the plasmid). Some restriction
digestions using these enzymes and two others (in alphabetical order), KpnI
and SacI, have been run. Neither KpnI nor SacI will cut in the
vector; they may or may not cut in the insert. You are to draw a restriction
map with APPROXIMATE sizes - actually, all you need to get right are the
RELATIVE sizes, along with the ORDERING of the sites (5 points for ordering,
5 points for relative sizes - but you are very unlikely to get the sizes
right if you get the ordering wrong!). Below are ranges for the sizes of the
bands produced in the restriction digestions - note that these are ranges,
not single numbers; again, you only need to give APPROXIMATE/RELATIVE sizes.

AbcI or XyzI alone:	12932-13995
AbcI and XyzI:		3941-4214	8774-9380
AbcI and SacI:		5147-5502	7679-8209
KpnI and SacI:			4504-4815	8208-8775
KpnI and XyzI:		5881-6287	7183-7680
SacI and XyzI:		1356-1450	11457-12248

Master:

Copy: 3
Order: AbcI - KpnI - SacI - XyzI
Size order: BC>Vector>AB>CD

A is AbcI
B is KpnI
C is SacI
D is XyzI